What is UV VIS diffuse reflectance spectroscopy?
UV–vis diffuse reflectance spectroscopy (DRS) is an especially useful technique to characterize the local titanium environment in titanosilicates [16]. Figure 3 depicts the UV–vis DRS of calcined sample D. Two absorption bands can be distinguished between 190–400 nm.
How do you measure diffuse reflection?
Relative diffuse reflectance is measured by using an integrating sphere to measure the diffuse reflected light with specular reflected light excluded, as shown in Fig. 4-1. This is used to measure samples with a rough surface, such as paper.
How do you use UV Probe software?
1) Push the F4 button on the UV-VIS instrument keypad. This will enable PC control. 2) Log into the UV-VIS software with your username and password. 3) After the UV-Probe software comes up, click on the “Connect” button near the bottom of the screen to connect with the spectrometer.
What is diffuse reflectance spectroscopy used for?
13.2. Diffuse reflectance spectroscopy (DRS) utilizes visible and near-infrared (NIR) light to determine the hemoglobin concentration and oxygenation of blood in superficial capillaries (Figure 13.9b; Douplik et al., 2014).
How do you convert %r to absorbance?
Data are reported as percentage of radiation reflected by the reference surface. Spectral Absorbance (A) was calculated as: Absorbance = 1- (Reflectance + Transmittance).
How do you calculate reflectance?
Reflectivity can be calculated as p(y) = Gr(y)/Gi(y) where p is the reflectivity, y is the wavelength of the light, Gr is the reflected radiation and Gi is the incident radiation. Calculate reflectance from the reflectivity. Reflectance is the square of the reflectivity so q(y) = (Gr(y)/Gi(y))^2.
What is K factor in spectrophotometer?
The subtraction A975 nm – A900 nm is called the “K-factor”, and it is simply used as a reference value that can be compared to the absorbance that is measured in microplates. Values of K-factor are available for many different aqueous buffers.
What is UV spectrophotometer auto zero?
This allows you to set a “0” so that your absorbance readings will have a basis to be compared to. Zero (blank) the SmartSpec 3000 by placing a cuvette containing only media (NO BACTERIA) into the cuvette chamber and pressing Read Blank. You can re-zero the instrument at any time during use.
What is diffuse reflectance FTIR?
Diffuse reflectance (DRIFTS, Diffuse Reflectance Infrared Fourier Transform Spectroscopy) is applied to analyze powders and rough surface solids. Typically, the technique is applied to powders since the technique relies upon scattering of radiation within the sample.
How do you calculate wavelength from absorbance?
This can be given as Ay = -log10(I/Io) where Ay is the absorbance of light with wavelength y and I/Io is the transmittance of the test material. Observe that absorbance is a pure number without units of measure. Absorbance is based on the ratio of two intensity measurements, so the resulting value has no units.