What is the most common cause of ABO discrepancies?
Technical/Clerical errors leading to ABO discrepancy were noticed in 12 (9.3%) cases. The most frequent cause of ABO discrepancies in forward grouping was subgroups of A Antigen (44.6%) and in reverse grouping was cold autoantibody (23.9%).
What is ABO forward typing?
Forward typing- A blood typing procedure whereby patient red blood cells are mixed with Anti-A and Anti-B reagents. Rh Immune globulin – Concentrated solution of Anti-D from human plasma which is given to preg- nant women who are Rh negative and who may be carrying and/or have delivered an Rh positive baby.
What are the four ABO discrepancies?
The table that follows provides four useful categories. Weak, missing reactions (including mixed field) in the forward (red cell) typing. Unexpected reactions in the forward (red cell) typing. Weak or missing reactions in the reverse (serum) typing.
What are the ABO discrepancies between forward and reverse grouping?
1.1 To resolve ABO and Rh blood group discrepancies. 1.2 Blood group discrepancies exist when the reaction in the forward group (red cells) does not match the reactions in the reverse grouping (plasma/serum), when expected reactions are weak or negative, or if the previous and current results do not match.
How do you resolve ABO Rh discrepancies?
In this situation, the discrepancy can be resolved by washing and re-suspending patient RBCs in saline, followed by repeat forward typing with anti-A and anti-B.
What is ABO discrepancies?
An ABO discrepancy exists when the result of an ABO RBC typing, or forward type, does not agree with the result of the plasma typing, or reverse type.
What is ABO discrepancy?
An ABO discrepancy exists when the result of an ABO RBC typing, or forward type, does not agree with the result of the plasma typing, or reverse type. It is extremely important in the clinical laboratory to record these initial discrepant results.
What is the difference between ABO forward typing for ABO reverse typing?
The forward grouping suggests the presence or absence of A and B antigens in RBCs, whereas reverse grouping indicates the presence or absences of anti-A and anti-B in serum.
What are the ABO discrepancies?
An ABO discrepancy exists when the result of an ABO RBC typing, or forward type, does not agree with the result of the plasma typing, or reverse type. This brief review examines several causes of ABO discrepancies encountered in the clinical transfusion service.
What are O cells?
The O-Cell is a hydraulically driven, high capacity, sacrificial loading device installed within the foundation unit. As the load is applied to the O-Cell, it begins working in two directions; upward against upper side shear and downward against base resistance and lower side shear (if applicable).
What are ABO discrepancies?
Why is slide method not recommended for reverse typing?
Slide test is quick and needs only simple equipment. It can be used in blood donation camps and in case of an emergency. However, it is not recommended as a routine test in blood banks since weakly reactive antigens on cells on forward grouping and low titer anti-A and anti-B on reverse grouping may be missed.