What is the genome of M13 bacteriophage?
Bacteriophage M13 has already been mentioned in Chapter 2. The genome of this phage consists of 6.4 kb of single-stranded, positive-sense, circular DNA and encodes 10 genes.
What is M13 plasmid?
M13 plasmids are used for many recombinant DNA processes, and the virus has also been used for phage display, directed evolution, nanostructures and nanotechnology applications. Escherichia virus M13.
What is M13 in biology?
a naturally occurring bacteriophage that contains a single-stranded circular genome of about 6400 Bases that infects only enteric bacteria containing the F-factor or producing the F pilus protein.
What is the size of the DNA molecule which can be packed by the bacteriophage M13 protein coat?
M13 is estimated to be approximately 900 nm in length and 7 nm in diameter, with a mass of approximately 16.3 MDa.
What is a bacteriophage and what does it do?
Bacteriophages are viruses that infect bacteria but are harmless to humans. To reproduce, they get into a bacterium, where they multiply, and finally they break the bacterial cell open to release the new viruses. Therefore, bacteriophages kill bacteria.
What is the nature of M13 phage DNA?
M13 phage is a bacterial virus composed of a single-stranded DNA encapsulated by various major and minor coat proteins. It has a long-rod filament shape that is approximately 880 nm in length and 6.6 nm in width (Smith & Petrenko, 1997).
What are the major coat proteins in M13 phage that are used in phage display?
The M13 DNA is coated by major coat protein pVIII which has 2,700 copies in the mature phage and has 50 amino acid residues. The pVIII is coded by a single gene with the same name (gVIII). The minor coat proteins include pIII and pVI at one end and pVII and pIX at the other end of the phage.
Which is a bacteriophage?
A bacteriophage is a type of virus that infects bacteria. In fact, the word “bacteriophage” literally means “bacteria eater,” because bacteriophages destroy their host cells. All bacteriophages are composed of a nucleic acid molecule that is surrounded by a protein structure.
How do bacteriophages work?
Bacteriophages kill bacteria by making them burst or lyse. This happens when the virus binds to the bacteria. A virus infects the bacteria by injecting its genes (DNA or RNA). The phage virus copies itself (reproduces) inside the bacteria.
What does the name bacteriophage mean?
A bacteriophage is a type of virus that infects bacteria. In fact, the word “bacteriophage” literally means “bacteria eater,” because bacteriophages destroy their host cells.
How is the DNA sequence of bacteriophage M13 determined?
Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd The 6407 nucleotide-long sequence of bacteriophage M13 DNA has been determined using both the chemical degradation and chain-termination methods of DNA sequencing.
What is the bacteriophage M13 used for?
The bacteriophage known as “M13” forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various “phage display” methodologies and “combinatorial” DNA and peptide libraries. M13 is a filamentous bacteriophage which infects E. coli host.
What is the genome of M13?
M13 is a filamentous bacteriophage which infects E. coli host. The M13 genome has the following characteristics: The gene VIII protein forms a tubular array of approx. 2,700 identical subunits surrounding the viral genome
What is the shape of M13 phage?
M13 phage is a bacterial virus composed of a single-stranded DNA encapsulated by various major and minor coat proteins. It has a long-rod filament shape that is approximately 880 nm in length and 6.6 nm in width ( Smith & Petrenko, 1997 ).