What is luciferase assay?
A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene.
How is luciferase assay done?
Cell debris is removed by microcentrifugation and luciferase activity is measured using a luminometer. Some luminometers directly inject the reagents into the cell lysate. Such automation allows the signal to be measured at a precise time following injection, which can increase the consistency of the results.
What is the purpose of luciferase?
The power of luciferase has been harnessed by scientists to devise reactions whose light output is used to monitor biological processes including gene expression, biomolecular binding, and cell viability.
How does a reporter assay work?
A bioluminescent reporter assay consists of both a luciferase reporter enzyme and a detection reagent that provides the enzyme substrate. When the reporter enzyme and detection reagent are combined, the light emitted is proportional to reporter gene expression levels and is detected using a luminometer.
Is Luc a reporter?
The ARE/LUCPoter(TM) reporter cell line is designed to monitor the induction of ARE and can be used for screening of agonists, antagonists or signaling inhibitors of ARE induction (Nrf2 activity) as well as for studying the Keap1/Nrf2 signaling pathways.
How long does luciferase last?
The luminescence signal decays over the course of about 10 minutes of reaction time, although signal half-life may vary depending on luciferase expression levels. The light production resulting from the luciferase reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface.
Is luciferase secreted?
Also, luciferases such as Gaussia, Metridia and Cypridina are naturally secreted and therefore have been used to study the mechanisms and regulation of the secretory pathway.
What is luciferase reporter?
Published September 22, 2021. The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously.
What is luminometer testing?
Luminometer. The luminometer evaluates surface cleanliness by measuring the chemical marker adenosine triphosphate (ATP), a compound found in all types of plant, animal and microbial cells. The science behind the luminometer is based on the enzyme luciferase – the same enzyme that makes firefly tails glow at night.