What is Lipofectamine RNAiMAX?
Lipofectamine™ RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments.
What is the difference between lipofectamine 2000 and RNAiMAX?
Lipofectamine® rnaiMaX reagent is designed specifically for the delivery of sirna and mirna while Lipofectamine® 2000 reagent delivers Dna or sirna with excellent transfection performance for protein expression, gene silencing, and functional assays.
How does Lipofectamine transfect cells?
Add the oligomer-Lipofectamine 2000 complexes to each well containing cells and medium. Mix gently by rocking the plate back and forth. Incubate the cells at 37°C in a CO2 incubator for 24-96 hours until you are ready to assay for gene knockdown. Medium may be changed after 4-6 hours.
Is Lipofectamine a transfection reagent?
Lipofectamine™ Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells. Lipofectamine™ Transfection Reagent is a trusted, value reagent with many years of cited use in thousands of publications and with many cell lines.
What is Oligofectamine?
Oligofectamine™ Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA (siRNA) into eukaryotic cells.
How do transfection reagents work?
Transfection reagents, such as Transfectamine™ 5000, are positively charged and attract the negatively charged DNA to form a positively charged polymer, which can interact with negatively charged cell membrane which enables the uptake of this polymer into the cell.
How do you transfect hek293t cells?
The protocol for a 24-well transfection reaction with HEK293 cells is here:
- Plate 10,000-15,000 HEK293 cells per well in 0.5 ml of complete growth medium 12-24 hours prior to transfection.
- Wash with 1xPBS and add 0.5 ml of fresh growth medium.
How Lipofectamine facilitates the entry of DNA into cells?
Lipid-mediated transfection relies on cationic lipids, such as lipofectamine, which facilitate DNA and mRNA delivery into cells. The cationic lipids form a unilamellar liposomal structure with a positive surface charge and fuse with nucleic acids to form a transfection complex.
What is Lipofectamine made of?
LIPOFECTIN is a 1:1 (w/w) mixture of N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) and dioleoylphosphatidylethanolamine (DOPE).
How are mammalian cells transfected?
The physical transfection methods are the most recent methods and use diverse physical tools to deliver nucleic acids. The methods include direct micro injection, biolistic particle delivery, electroporation, and laser-based transfection [13].