What is capture seq?
Capture-Seq is a targeted next-generation sequencing (NGS) platform for characterizing between 1,000 and 450,000+ targets in any species (including complex polyploids). Samples may be comprised of a single species, a mix of closely related species or distantly related taxa.
What is cDNA in biology?
Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase, a DNA polymerase that can use either DNA or RNA as a template. From: Encyclopedia of Genetics, 2001.
What is targeted RNA seq?
Targeted RNA-sequencing (RNA-Seq) is a highly accurate method for selecting and sequencing specific transcripts of interest. It offers both quantitative and qualitative information.
Does open reading frame include start codon?
A nucleotide sequence that contains a start codon (initiation codon, typically AUG) and a stop codon (termination codons, UAA, UAG or UGA) is called an open reading frame (ORF). An ORF is hence a portion of an organism’s genome which contains a sequence of bases that could potentially encode an individual protein.
What is cDNA used for?
cDNA is often used to clone eukaryotic genes in prokaryotes. When scientists want to express a specific protein in a cell that does not normally express that protein (i.e., heterologous expression), they will transfer the cDNA that codes for the protein to the recipient cell.
What is an isoform in genetics?
Gene isoforms are mRNAs that are produced from the same locus but are different in their transcription start sites (TSSs), protein coding DNA sequences (CDSs) and/or untranslated regions (UTRs), potentially altering gene function.
What is targeted gene expression?
Targeted gene expression analysis with qPCR aims at the quantitative measurement of mRNA expression of defined target genes (e.g. in different genotypes, prior to and during drug therapy or after the administration of compound candidates in the pre-clinical phase of drug development).
What is RNA-Seq Illumina?
Introduction to RNA Sequencing RNA-Seq allows researchers to detect both known and novel features in a single assay, enabling the detection of transcript isoforms, gene fusions, single nucleotide variants, and other features without the limitation of prior knowledge.
Why are there 3 open reading frames?
Genetic code During transcription, the RNA polymerase read the template DNA strand in the 3′→5′ direction, but the mRNA is formed in the 5′ to 3′ direction. The mRNA is single-stranded and therefore only contains three possible reading frames, of which only one is translated.
Does ORF include stop codon?
An open reading frame, as related to genomics, is a portion of a DNA sequence that does not include a stop codon (which functions as a stop signal).