What is a sep-pak?

What is a sep-pak?

Column liquid chromatography is used in industry to separate mixtures and detect trace components of a mixture. The Sep-Pak cartridge is filled with a solid nonpolar material that helps to separate components of mixtures. Sep-Pak cartridges are reusable if cleaned and stored properly after use.

What is a Sep-Pak made of?

Selected Waters Sep-Pak sorbents are available in a unique, multi-capacity, award- winning 96-well plate format designed by Waters. Plates are molded from high-purity polypropylene. Wells are filled with the same high quality sorbents and frit materials available in the respective cartridge formats.

What is Sep-Pak C18?

Sep-Pak C18 Vac cartridges contain a hydrophobic, reverse-phase, silica-based bonded phase that is used to adsorb analytes of even weak hydrophobicity from aqueous solutions. The Sep-Pak C18 sorbent is made using the most reproducible bonding technology available, utilizing monofunctional silane chemistry.

How do you use a Sep-Pak C18 cartridge?


  1. Condition with six to ten holdup volumes of methanol.
  2. Equilibrate with six to ten holdup volumes of water.
  3. Load a sample diluted in water.
  4. Wash with three to six holdup volumes of water.
  5. Elute with three times (3X) the holdup volume of methanol.

How does Sep Pak work?

What is C18 cartridge?

The C18 solid phase extraction (SPE) cartridge uses octadecyl silica as a filler to retain non-polar compounds by strong hydrophobic interaction. The filler retains most of the organic matter and is widely used in the fields of environmental and food safety.

What dyes are in grape Kool Aid?

The ingredients of grape Kool-Aid are sugar, citric acid, ascorbic acid, blue dye, and red dye.

How does reverse phase chromatography work?

Reversed-phase chromatography employs a polar (aqueous) mobile phase. As a result, hydrophobic molecules in the polar mobile phase tend to adsorb to the hydrophobic stationary phase, and hydrophilic molecules in the mobile phase will pass through the column and are eluted first.

Why do you think the blue dye did not elute in the 5% isopropanol while the red dye did elute?

The blue dye, however, is more non-polar than the red dye and is still attracted more strongly to the column than it is attracted to the solvent. Therefore, only the red dye is eluted from the sample by the 5% isopropyl alcohol solution.

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