What does THP-1 cells stand for?
THP-1 designates a spontaneously immortalized monocyte-like cell line, derived from the peripheral blood of a childhood case of acute monocytic leukemia (M5 subtype) (3).
What are THP-1 monocytes?
THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research. Homo sapiens, human.
Are THP-1 macrophages adherent?
Namely, increased phagocytic activity, HLA-DR expression and MLR stimulator activity were remarkable. This adherent cell line was designated as activated-THP-1 (A-THP-1), since it demonstrated characteristics of activated macrophages continuously without exogenous stimulation.
How do you count THP-1 cells?
THP-1 cells are counted using a haemocytometer a cell suspension with a concentration of 1 × 105 cells/ml is made up. One 6 well-plate is then prepared by adding 2 ml of RPMI/FCS media per 6 well. The transwell inserts are then placed into the 6 well containing the 2 ml medium.
Are THP-1 cells adherent?
THP-1 cells are non-adherent, so all treatments you perform to promote their attachment are going to have an impact on them. For instance, PMA tends to upregulate the expression of some genes in differentiated macrophages, which could affect the gene expression induced by other stimuli.
What are THP-1 derived macrophages?
THP-1 cells can be differentiated into macrophage-like cells that resemble properties of mature macrophages by activation of protein kinase C (PKC) with phorbol-12-myristate-13-acetate (PMA), ultimately resulting in cells with increased adherence and loss of proliferative activity (Schwende et al., 1996).
How are THP-1 cells different?
The conditions used to differentiate THP-1 cells are highly variable with PMA concentrations ranging from 10 to 400 ng/mL and the duration of treatment ranging from 1–5 days of continuous stimulation, which may be followed by at least 1 day of rest.
Are THP-1 cells macrophage?
Why do THP-1 cells clump?
Popular Answers (1) THP-1 cells grow in clumps as when they divide the new cell attaches to the one it split from. With my THP-1 gentle pipetting up and down will break the clumps but the cells remain viable.
Are THP-1 cells immortal?
The short answer is no, the differentiated macrophages do not retain immortality. In my experience, when THP-1 cells are induced with PMA, they will stop proliferating.
Are THP-1 cells phagocytic?
Results. To identify genes regulating phagocytosis of the bacterium S. aureus, we selected the human monocytic cell line THP-119 as our primary screening model. THP-1 cells have been shown to spontaneously phagocytose bacteria without the need for activation or differentiation20.
How do subcultures THP-1 cells?
Routinely THP-1 cells are cultured in T75 flasks in 20 ml complete cell culture medium. They are subcultured every three to four days (twice a week) at a concentration of 8×105 to 1×106 cells/ml. This situation is achieved by seeding 5×105 to 1×106 cells per flask (equaling 2.5×104 to 1×105 cells/ml, respectively).