What is the molecular weight of DNase?
44-60 kDa
Molecular Weight (observed) of DNase l: 44-60 kDa.
How do you resuspend DNase?
Reconstitute DNase I, recombinant in water. This is the most common solvent used. Store the solution for 2 days at +2 to +8°C or 1 month in aliquots at −15 to − 25°C. Avoid repeated freezing and thawing.
Does DNase I need magnesium?
DNase I requires activation through divalent metals; maximum activation is achieved with magnesium and calcium but manganese, cobalt, and zinc may also be used. If random cleavage or nicking is desired, magnesium ions should be used as an activator.
How long is DNase stable at room temperature?
The DNase Max Kit is stable at room temperature (15–25°C) for up to 6 months or at 2–8°C for 2 years with no loss of activity. Room temperature storage eliminates the need to aliquot and freeze stocks of DNase I enzyme and removes concerns about decreased enzyme activity due to freeze–thaw cycles.
How do you dissolve DNase 1?
Dissolve 2 mg of crude pancreatic DNase I (Sigma or equivalent) in 1 ml of 50 mM NaCl, Tris-Cl (pH 7.5), 1 mM MgCl2. When the DNase I is dissolved, add 1 ml of glycerol to the solution and mix by gently inverting the closed tube several times. Take care to avoid creating bubbles and foam.
What is DNase 1 and why is it in units ml?
Unit definition: 1 unit is defined as the amount of enzyme required to produce an increase in absorbance at 260nm of 0.001/min/mL at 25°C of highly polymerized DNA. For Research Use Only. Not for use in diagnostic procedures.
What is Benzonase used for?
Benzonase® endonuclease is standardly used in DNA digestion in R&D laboratories. Researchers found that Benzonase® endonuclease is not only working in small R&D scale but has the probability to be scaled up to industrial virus purification in vaccine and viral vector manufacturing.
What is DNase agar used for?
DNase agar is a differential medium that tests the ability of an organism to produce an exo-enzyme, called deoxyribonuclease. DNase are extracellular endonucleases that cleave DNA and release free nucleotides and phosphate. DNase agar contains nutrients for the bacteria, DNA, and mostly methyl green as an indicator.
What are the enzyme impurity levels for d4527 and d5025?
The enzyme impurity levels for each, however, are different. D5025 has the following impurity specification: Chymotrypsin (<0.5%), Protease (<0.05%), and RNase (<0.02%). The impurity specifications for D4527 are: Chymotrypsin (<0.01%), Protease (<0.005%), and RNase (<0.01%).
What is the function of DNase 1?
DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random.
How does DNase I work with Mn2+?
In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme.
What is the pH of Mn2+ in DNA?
Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion.