What is PE labeled antibody?
Phycoerythrin (PE) is a type of phycobiliproteins found in cyanobacteria and red algae. PE-conjugated antibodies are broadly used for flow cytometry and immunofluorescence microscopy.
How do you label an antibody?
Two types of labeling methods are commonly used depending on what part of the antibody is labeled. The first is to label the amino groups (NH2 groups) of the antibody (the NH2 type), and the second is to label the thiol groups (SH groups) (the SH type). Each method has advantages and disadvantages.
What are conjugation kits?
Lightning-Link® Conjugation Kits are designed for labeling antibodies with one type of label (single labeling). All kits target the same chemical groups on the antibody (free amines).
How do you conjugate fluorophore to antibody?
Fluorochromes can be covalently conjugated to antibodies through reactions with thiol or amine groups. Typically, fluorochromes containing isothiocyanate, succinimidyl ester, or sulfonyl chloride reactive groups are conjugated to amines on the antibody molecules.
What is FITC conjugate?
Streptavidin-FITC conjugate is typically used in the immunodetection of biotinylated proteins. This conjugate is suitable for use in immunomicroscopy and fluorescence-activated cell sorting and analysis.
How does antibody labeling work?
Antibody labeling can be done by two methods: Direct labeling and indirect labeling. As the name implies direct labeling uses a single primary antibody for the labeling process. This antibody is covalently bonded to a labeling molecule. Then the primary antibody is added to the antigen complex and incubated.
What are labeled antibodies used to detect?
Labelled antibodies are used to detect the presence of proteins in Western blotting.
What is fluorescein sodium used for?
Fluorescein sodium, the sodium salt of fluorescein, is used extensively as a diagnostic tool in the field of ophthalmology and optometry, where topical fluorescein is used in the diagnosis of corneal abrasions, corneal ulcers and herpetic corneal infections.