What is morpholino zebrafish?
Morpholino antisense oligonucleotides and mechanisms of gene knockdown. The main antisense chemistry used in zebrafish are morpholino phosphorodiamidate oligonucleotides (morpholinos, MOs). 11. MOs are composed of a phosphorodiamidate backbone with a morpholine ring and the same bases as DNA (A).
What is morpholino knockdown?
One of the most rapid and economical techniques for performing reverse genetic analysis is the powerful approach employing morpholino-modified anti-sense oligonucleotides (MOs), which provide transient gene knockdown by binding to RNA and inhibiting protein synthesis (5).
How do Morpholinos block translation?
Translation blocking morpholinos are modified antisense oligomers, typically of 25 bp targeted to the start codon of the gene of interest. They block translation through steric interference with the translation initiation complex and thus prevent loading of the mRNA into ribosomes for translation.
Why use Morpholinos?
Morpholinos are typically used to block translation of mRNA and to block splicing of pre‐mRNA, though they can block other interactions between biological macromolecules and RNA. Morpholinos are effective, specific, and lack non‐antisense effects.
What is a vivo morpholino?
A Vivo-Morpholino is comprised of a Morpholino oligo with a unique covalently linked delivery moiety, which is comprised of an octa-guanidine dendrimer. It uses the active component of arginine rich delivery peptides (the guanidinium group) with improved stability and reduced cost.
What are antisense morpholino oligonucleotides?
Morpholinos are synthetic antisense oligonucleotides (around 25 nucleotides) designed to bind and block the translation initiation complex of messenger RNA (mRNA) sequences. This technology has been used to test the role of specific genes by transient blocking, particularly during development.
How do Morpholinos work?
Morpholinos. Morpholinos are synthetic antisense oligonucleotides (around 25 nucleotides) designed to bind and block the translation initiation complex of messenger RNA (mRNA) sequences. This technology has been used to test the role of specific genes by transient blocking, particularly during development.
How do morpholino oligonucleotides work?
Does siRNA inhibit transcription?
Recently, in human cells small-interfering RNAs (siRNAs) have been shown to mediate transcriptional gene silencing (TGS). The observation that siRNAs can function to suppress gene expression at the level of transcription has created a major paradigm shift in mammalian RNA interference.