What is hydrodynamic transfection?
Hydrodynamic transfection uses a hydrodynamic force produced by the pressurized injection of a large volume of DNA solution into the blood vessel, which permeabilizes the capillary endothelium and generates pores in the plasma membrane of the surrounding parenchyma cells.
How do you inject mouse tail veins?
Insert the needle (small gauge, 27-30 for mice and 25-27 for rats), bevel up, into the vein towards the direction of the head. Keep the needle and syringe parallel to the tail. Aspiration is not advised as it may cause the vein to collapse, but a flash of blood in the hub of the needle may be seen when first placed.
What is hydrodynamic injection?
The hydrodynamic tail vein injection is a technique that is used to deliver nucleic acids into live mice. Delivery through this method results in the in vivo transfection of foreign DNA primarily in the liver.
What is HTV injection?
Hydrodynamic tail vein (HTV) delivery is highly efficient at transferring nucleic acids to the liver of mice and rats. This simple non-viral gene transfer procedure entails the rapid delivery of naked plasmid DNA (pDNA) in a relatively large volume of physiological solution.
What is the maximum volume for intravenous injection?
vessel. When substances are given by IV infusion, the volume should not exceed 5% of the circulating blood volume over 2 hours or 4 ml/kg/h. 4. The maximum recommended injection volume for a dosing solution that is given rapidly IV is 1 ml/kg body weight for most laboratory animal species.
How does hydrodynamic tail vein injection work?
How does hydrodynamic sample injection work in capillary electrophoresis?
Hydrodynamic injection is accomplished by the application of a pressure difference between the two ends of a capillary. The amount of sample injected can be calculated by the Poiseuille equation.
What is the route of injection for lab animals?
There are five commonly used routes of parenteral administration: subcutaneous (SC/SQ), intraperitoneal (IP), intravenous (IV), intradermal (ID), and intramuscular (IM).
How does hydrodynamic injection work?
Hydrodynamic gene delivery uses a hydrodynamic force generated by a pressurized injection of a large volume of DNA solution into the blood vessel so as to permeabilize the capillary endothelium and generate “pores” in the plasma membrane of the surrounding parenchyma cells, through which DNA or other macromolecules of …
What are the advantages of hydrodynamic injection over electrokinetic introduction?
The advantage of hydrodynamic injection is that the quan- tity of individual analytes injected is constant, independent of their own mobility, in contrast to electrokinetic injection. This mode is mechanically the simplest one and adequate for samples with a low viscosity. and solution viscosity.
Where do you inject mice?
Mice & Rats -Intramuscular Injection (IM) IM injections are administered in the thigh muscles of the hind limb. Caution must be used to avoid injury to the sciatic nerve, which is located in close proximity to the femur.