What happens when a specimen is hemolyzed?

What happens when a specimen is hemolyzed?

The term hemolysis designates the pathological process of breakdown of red blood cells in blood, which is typically accompanied by varying degrees of red tinge in serum or plasma once the whole blood specimen has been centrifuged.

What does it mean when it says specimen slightly hemolyzed?

A hemolyzed test result or ‘haemolysis’ can happen when the red blood cells in the sample burst. When this happens, it is usually not possible to get a test result.

Why are hemolyzed specimens unacceptable for testing?

Common Causes of Unacceptable Specimens or Inaccurate Test Results: Hemolysis Lysis of red blood cells during collection or handling of blood specimens may cause hemoglobulin or other components to escape from the cells into the serum or plasma.

How do you prevent a hemolyzed blood sample?

Best Practices to Prevent Hemolysis

  1. Use the correct needle size for blood collection (20-22 gauge).
  2. Avoid using butterfly needles, unless specifically requested by patient.
  3. Warm up the venipuncture site to increase blood flow.
  4. Allow disinfectant on venipuncture site to dry completely.

Why are hemolyzed samples rejected?

In some reports, hemolyzed specimens, the most common reason for rejection, account for ∼60% of rejected specimens, fivefold more than the second most common cause (2)….To the Editor.

. n %
Infusion access into syringe 58 11.5
Catheter partially obstructed 35 6.9
Blood forced into the tube 26 5.1
In vivo hemolysis 9 1.8

What are the reasons for hemolysis?


  • chemicals.
  • infections.
  • medicines such as penicillin, acetaminophen, quinidine, rifampin, heparin, and clopidogrel.
  • any condition that causes increased spleen activity.
  • immune reactions, such as autoimmune hemolytic anemia (AIHA) and incompatible blood transfusion.
  • intense physical exercise.

How does hemolysis happen?

When does hemolysis occur? Certain medical conditions can result in blood hemolysis such as hemolytic anemia, liver disease or a transfusion reaction. However, most hemolysis occurs because of procedural errors during the pre-analytical phase of specimen collection, processing and transport.

Are hemolyzed specimens acceptable?

(6) They concluded that testing hemolyzed samples and reporting results for BUN, creatinine, amylase, and lipase was acceptable while testing and reporting phosphorus, sodium, potassium, total protein and LDH provided results that were unacceptably compromised.

Can hemolyzed blood be tested?

Hemolysis may also be due to pathological conditions, such as immune reactions, infections, medications, toxins and poisons, and therapeutic procedures. Frequently, laboratory testing can still be performed on a hemolyzed sample.

What sample is most likely to be hemolyzed?

Which specimen is more likely to be hemolyzed, an evacuated tube drawn specimen or a syring drawn specimen? why? A syringe drawn specimen because of the higher risk for trauma during the transfer of the specimen from syringe to tube and the delay before the blood is mixed with the anti-coagulant.

Which of the following test is most seriously affected by hemolysis?

Lactate dehydrogenase appeared to be most sensitive to hemolysis; the increase of č 1000 U of lactate dehydrogenase per liter resulted in a 4.5-fold higher enzyme activity at 4.5 g of hemoglobin per liter of plasma than at 0.27 g/L.

How does hemolyzed specimen affects the test result?

Certain lab tests can be affected and the reported results will be inaccurate. It falsely decreases values such as RBC’s, HCT, and aPTT. It can also falsely elevate potassium, ammonia, magnesium, phosphorus, AST, ALT, LDH and PT.

Does re-collection of hemolyzed blood specimens delay patient care in emergency departments?

Introduction: Re-collection of hemolyzed blood specimens delays patient care in overcrowded emergency departments. Our emergency department was unable to meet a benchmark of a 2% hemolysis rate for the collection of blood samples.

What does hemolyzed mean in a lab report?

When lab reports indicate that a blood specimen has been hemolyzed, then this is an indication that the red blood cells in that sample were destroyed in some way. The destruction of the red blood cells will release hemoglobin and this renders the sample useless.

Do hemolysis rates of blood specimens differ by blood collection techniques?

Our hypothesis was that hemolysis rates of blood specimens differ dependent on the blood collection technique by venipuncture or intravenous catheter draw. Methods: A prospective, cross-over study of blood collection techniques in a 64,000 annual visit, community teaching hospital emergency department was conducted.

Why do nurses draw blood with an intravenous line instead of needles?

The availability of an intravenous line already placed pushes many nurses to use this route for blood drawing, even if it is known that this technique is associated with an increased rate of hemolysis compared to blood sampling with a needle.

Begin typing your search term above and press enter to search. Press ESC to cancel.

Back To Top