What does Aurora b kinase do?
Aurora B is a mitotic checkpoint kinase that plays a pivotal role in the cell cycle, ensuring correct chromosome segregation and normal progression through mitosis. Aurora B is overexpressed in many types of human cancers, which has made it an attractive target for cancer therapies.
How is Plk1 activated?
Like many kinases, Plk1 is activated by phosphorylation in its T loop, which induces a conformational change to promote catalysis. Phosphorylation at that site is also thought to lock the protein in an open conformation, where the kinase domain and the PBD are dissociated.
Where is Aurora b kinase located?
Aurora B is a chromosomal passenger protein. Specifically, Aurora B localizes to the chromosomes in prophase, the centromere in prometaphase and metaphase, and the central mitotic spindle in anaphase. This localization has been determined by indirect immunofluorescence in mammalian, C. elegans, and Drosophila cells.
How does Aurora B regulate chromosome alignment?
Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores.
What does polo kinase do?
The Polo Kinase is a central regulator of cell division required for several events of mitosis and cytokinesis. In addition to a kinase domain (KD), Polo-like kinases (Plks) comprise a Polo-Box domain (PBD), which mediates protein interactions with targets and regulators of Plks.
What is chromosome orientation?
The process of establishing and maintaining the proper bioriented attachment of chromosomes to spindle microtubules is complex, and it frequently proceeds through intermediate stages of inappropriate attachment (Fig.
What are the 8 steps of cell cycle?
The stages of the cell cycle (interphase, prophase, metaphase, anaphase, telophase) can be remembered by using the mnemonic IPMAT. During anaphase the chromatids are pulled to opposite poles of the cell by the shortening of the spindle fibres….Mitotic Phase (ESG5M)
- prophase.
- metaphase.
- anaphase.
- telophase.
- cytokinesis.