What do Caco-2 cells do?
6.5. Caco-2 cells have special features: they develop into small intestinal cells with tight junctions and microvilli, expressing the drug metabolizing enzymes involved in uptake of the agents crucial in drug metabolism [22,23].
What type of cells are Caco-2?
Caco-2 [Caco2] are epithelial cells isolated from colon tissue derived from a 72-year-old, White, male with colorectal adenocarcinoma. This cell line is a suitable transfection host and has applications in cancer and toxicology research.
How do you differentiate Caco-2 cells?
Standard methods used to differentiate Caco-2 cells involve seeding them on culture inserts fitted with polycarbonate filters, and allowing spontaneous differentiation to proceed for two to three weeks in culture medium containing 10% or 20% foetal bovine serum (FBS) in both the apical (AP) and basolateral (BL) …
Do Caco-2 cells produce mucus?
However, Caco-2 cells are mostly reported as non/low-mucus producing cells and do not or hardly express mucin-related genes, such as MUC-2 and MUC-625.
What do enterocytes do?
In the small intestine, the amount of enterocytes is about 80 % of the total epithelial cells. The main function of enterocytes is to absorb molecules from the gut lumen and their transport toward the surrounding connective tissue and blood vessels.
Where are Caco-2 cells from?
The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with many properties typical of absorptive enterocytes with brush border layer as found in the small intestine.
What is Caco-2 Transwell assay?
Culturing Caco-2 cells in Transwell plates is considered the reference in vitro technique to evaluate intestinal permeability in novel orally administered compounds, according to leading regulatory agencies (FDA, EMA and PMDA).
What does Caco-2 stand for?
human colorectal adenocarcinoma cells
Caco-2 is an immortalized cell line of human colorectal adenocarcinoma cells. It is primarily used as a model of the intestinal epithelial barrier. In culture, Caco-2 cells spontaneously differentiate into a heterogeneous mixture of intestinal epithelial cells.
What are the mucus producing cells?
Goblet cells are specialized cells equipped with specific biological machinery for the secretion of mucus and are present throughout the entire length of the intestine (Figure 2).
What are HT 29 cells?
HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. These cells are sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which are standard treatment options for colorectal cancer.
Do enterocytes produce enzymes?
Enterocytes, or intestinal absorptive cells, are simple columnar epithelial cells which line the inner surface of the small and large intestines. A glycocalyx surface coat contains digestive enzymes….
What can enterocytes absorb?
Enterocytes in the small intestine absorb large amounts of sodium ion from the lumen, both by cotransport with organic nutrients and by exchange with protons.