Is Enterobacter aerogenes SIM positive or negative?
Klebsiella aerogenes, previously known as Enterobacter aerogenes, is a Gram-negative, oxidase negative, catalase positive, citrate positive, indole negative, rod-shaped bacterium. The bacterium is approximately 1-3 microns in length, and is capable of motility via peritrichous flagella.
What is the colony morphology of Enterobacter aerogenes?
MICROSCOPIC APPEARANCE
| Gram Stains: | Negative. |
|---|---|
| Morphology: | Straight rods. |
| Size: | 0.6-1.0 micrometers by 1.2-3.0 micrometers. |
| Motility: | Some are motile by four to six peritrichous flagella. |
| Spores: | No. |
How do you identify Enterobacter aerogenes?
The next method used was a Urea test. This test consisted of stirring the bacterial growth into a tube of phenol red and urea to test for the presence of acid. After incubation the broth was still a yellow color, giving a negative result. This confirmed that Enterobacter aerogenes was the gram-negative bacterium.
Does Enterobacter aerogenes produce indole positive?
E. coli is indole-positive; Enterobacter aerogenes is indole- negative. Glucose is the major substrate oxidized by enteric bacteria for energy production. The end products of the oxidation process vary depending on the specific enzymatic pathways in the bacteria.
Is Enterobacter aerogenes VP positive?
Note: A culture will usually only be positive for one pathway: either MR+ or VP+. Escherichia coli is MR+ and VP-. In contrast, Enterobacter aerogenes and Klebsiella pneumoniae are MR- and VP+. Pseudomonas aeruginosa is a glucose nonfermenter and is thus MR- and VP-.
How do you identify Klebsiella aerogenes?
To differentiate Klebsiella aerogenes from common Salmonella enterobacteria and Klebsiella pneumoniae species, the isolates were injected with Kligler’s beveled agar, which included glucose, lactose, iron sulfate and the phenolic red indicator.
What is the normal habitat for Enterobacter aerogenes?
E. aerogenes is generally found in the human gastrointestinal tract and does not generally cause disease in healthy individuals. It has been found to live in various wastes, hygienic chemicals, and soil.
What does a positive MR test mean?
Interpretation. MR: If the tube turns red, the test is positive for mixed acid fermentation (one or more organic acids formed during the fermentation of glucose).
What does a positive VP test look like?
ər/ or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown color indicates a negative result.