How many types of pumps are there in HPLC?
There are 3 main types of HPLC pumps: 1. reciprocating pump – 90% usage. The solvent is drawn into a small chamber (when the solvent check valve is open) and pumped out of it (when the column check valve is open) by the back and forth motion of a motor-driven piston (Fig 28-6 or R5 Fig 6.9).
Which pump is most commonly used high-pressure pump in HPLC?
Binary Pumps (two solvent gradient formation) are the most popular HPLC pumps on the market. Binary pumps are typically high-pressure mixing pumps that have gradient formation of two solvents.
What is the unit of pump pressure in HPLC?
Operating pressure limits for regular HPLC systems (not UHPLC) are often in the range of 5800 – 7000 psi (400 – 500 bar), which is usually also appropriate for HPLC column hardware and (fully porous particle) packing material.
Why pumps are used in HPLC?
The purpose of the pump is to force the liquid through the HPLC system while it maintains a constant flow of mobile phase. This occurs regardless of the back pressure caused by the flow resistance of the HPLC column.
Why pump is used in HPLC?
An HPLC pump is also called a “solvent delivery system.” The purpose of the pump is to maintain a constant flow of mobile phase through the HPLC system. This is accomplished regardless of the back pressure caused by the flow resistance of the HPLC column.
Which of the given pump is used in HPLC?
D. Reciprocating (Constant Flow) Pumps It is the most generally used pump as it is economical and allows a wide range of flow rates. There is no limit on the reservoir size or operating time with this pump as is commonly found with other pumps.
What type of pump is used in HPLC?
There are two common types of HPLC pumps: reciprocating pumps and syringe pumps. Reciprocating pumps are by far the most common pump used and can have single piston or dual pistons.